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2.3. Lycopene estimationThe standard method of estimation of lycopene is spectrophotometric(Davis
1949). The absorbance of lycopeneextracted in petroleum ether (40 ml) was noted at three differentwavelengths
viz. 445
472
502. The absorbance atmaximum wavelength (k max) of 472 nm for lycopene inpetroleum ether was considered. The amount of lycopene(mg) was calculated by a specific extinction coefficient(E1%1 cm
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